检索范围:
排序: 展示方式:
Mature cystic teratoma of the parotid gland: a case report and review of the literature
Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 503-506 doi: 10.1007/s11684-009-0076-8
关键词: teratoma parotid gland
Endoscopic resection of a huge Brunner's gland adenoma
ZHANG Binbin, REN Xu, TANG Xiufen, CHI Yuxin, SHI Xuesong
《医学前沿(英文)》 2008年 第2卷 第4期 页码 414-418 doi: 10.1007/s11684-008-0080-4
关键词: superior Histological examination endoscopy gastro-intestinal gastrointestinal haemorrhage
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 345-353 doi: 10.1007/s11684-013-0282-2
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various advantageous properties, including self-renewal, extended proliferation potential, multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions. However, little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs. In this study, we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media (ASFCM) in order to determine their potential for differentiation into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions, and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs—positive for CD29, CD44, CD90, and CD105, and negative for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions. Like normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA and EDAR) that are characteristic of normal sweat gland cells. Taken together, our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes.
关键词: umbilical cord mesenchymal stem cells sweat gland preclinical
标题 作者 时间 类型 操作
Mature cystic teratoma of the parotid gland: a case report and review of the literature
Lenan SHAO DMD, MD, Hanxiong GUAN MD, Jie WAN MS,
期刊论文
Endoscopic resection of a huge Brunner's gland adenoma
ZHANG Binbin, REN Xu, TANG Xiufen, CHI Yuxin, SHI Xuesong
期刊论文